Test Code LEGION Culture, Legionella

Specimen Type
bronchial, sputum, lung, tissue
Specimen Requirement
Sputum: Submit entire collection in a sterile container.
Bronchial wash, lavage: 5-7 ml in sterile container. Avoid contaminating bronchoscope with tap water.
Pleural fluid: Submit entire collection in a sterile container.
Tissue: 1 gram in sterile container without fixatives or preservatives.
Add enough sterile saline to prevent desiccation. Do not wrap in gauze or send on a swab.
Rejection Criteria
Reject if specimen container is unlabeled or leaking.
Reject for inappropriate storage or transport.
Storage and Transport
Transport specimens to the Microbiology Lab within 1 hour at room temperature.
For transport times > 1 hour, store at 2-8 C and transport at 2-8 C within 24 hours.
Testing Schedule
Performed daily
Reference Range
No Legionella identified.
Method
Culture, Direct Fluorescent Antigen
CPT Code
87081
87140
87278
Performing Laboratory
Culture is performed at the North Dakota Public Health Laboratory.