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Test Code LEGION Culture, Legionella

Infectious

Specimen Type

bronchial, sputum, lung, tissue

Specimen Requirement

Sputum: Submit entire collection in a sterile container.

 

Bronchial wash, lavage: 5-7 ml in sterile container. Avoid contaminating bronchoscope with tap water.

 

Pleural fluid: Submit entire collection in a sterile container.

 

Tissue: 1 gram in sterile container without fixatives or preservatives.

Add enough sterile saline to prevent desiccation.  Do not wrap in gauze or send on a swab.

Rejection Criteria

Reject if specimen container is unlabeled or leaking.

Reject for inappropriate storage or transport.

Storage and Transport

Transport specimens to the Microbiology Lab within 1 hour at room temperature.

For transport times > 1 hour, store at 2-8 C and transport at 2-8 C within 24 hours.

Testing Schedule

Performed daily

Reference Range

No Legionella identified.

Method

Culture, Direct Fluorescent Antigen

CPT Code

87081

87140

87278

Performing Laboratory

Culture is performed at the North Dakota Public Health Laboratory.