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Test Code LAB923 Bordetella pertussis, PCR

Infectious

Specimen Type

Nasopharyngeal swab in universal transport media

Specimen Requirement

Nasopharyngeal swab:

1. Remove excess excretions from anterior nares.

2. Incline the patient’s head back.

3. Gently insert the mini-tip flexible flocked swab straight back. Keep the swab near the septum floor of the nose while gently pushing the swab into the posterior nasopharynx until resistance is felt.

4. Rotate the swab 2-3 times and hold in place for 5 seconds and remove from nostril carefully.

5. Place swab in the tube of universal transport media.

6. Label the tube with 2 patient identifiers, specimen source, time and date of collection, and collector initials.

 

Nasopharyngeal swab specimen collection posters are available upon request from the Microbiology Lab.

Rejection Criteria

Reject if specimen container is unlabeled.

Reject for inappropriate storage or transport.

Storage and Transport

Store refrigerated and transport within 4 days.

Testing Schedule

Performed daily (Monday – Friday)

Reference Range

Negative for Bordetella pertussis

Negative for Bordetella parapertussis

Method

nucleic acid test by helicase dependent amplification

CPT Code

87798