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HelpTest Code LAB5001 Culture, Brucella
Infectious
Specimen Type
Blood, Bone Marrow, other sterile body fluids
Specimen Requirement
Blood, bone marrow, other sterile body fluid:
Submit specimen in blood culture bottle.
Brucella Culture Volume Collection Guidelines:
optimal volume is critical for optimal test performance.
|
Age |
Optimum Total Volume |
Plus Aerobic/F |
Peds Plus/F |
|
10 years to adult |
10 ml |
10 ml |
NA |
|
6-10 years |
5 ml |
5 ml |
NA |
|
2-6 years* |
3 ml |
|
3 ml |
|
2 mo – 2 yr |
2 ml |
|
2 ml |
|
0 – 2 mo |
1-2 ml |
|
1-2 ml |
|
Neonate in NICU |
0.5 ml |
|
0.5 ml |
|
Max blood volume/bottle |
|
10 ml |
3 ml |
*Typically 1 ml per year of age.
Note: If Aerobic/Anaerobic and Brucella Cultures are ordered, the Aerobic bottle can be shared with both orders.
Tissue: Order an aerobic culture with a request to screen for Brucella.
Submit tissue biopsy (pea size) in a sterile container.
Rejection Criteria
Reject if specimen bottle is unlabeled, broken, or leaking.
Reject for inappropriate storage or transport.
Storage and Transport
Blood and body fluids: Transport cultures to the Microbiology Lab within 2 hours at room temperature.
If transport of blood culture bottles is delayed, specimens may be stored at room temperature for up to 48 hours before testing.
Tissue: Transport to the laboratory as soon as possible.
If transport is delayed, store at room temperature for up to 24 hours.
Testing Schedule
Performed daily
Reference Range
No growth in 10 days.
Testing Department
Microbiology
Method
Culture
CPT Code
87081
Turnaround Time
Preliminary Result 24 hours
STAT Turnaround Time
Not Applicable