Test Code LAB250 Gram Stain

Specimen Type
Tissue, fluid, respiratory or swab specimen
Specimen Requirement
Note: a descriptive anatomic source is required
Body fluid: at least 0.5 ml Submit entire collection in a sterile container. Do not submit a swab dipped in the fluid.
Tissue: at least 1 gram (pea size) Submit entire collection in a sterile container. Add a few drops of sterile, non-bacteriostatic saline to prevent desiccation.
Swab: red-topped double swab culturette (1 swab is used for Gram stain, the other for aerobic culture) or
white-topped single swab culturette (if no Gram stain needed). Collect swab specimen after decontaminating wound. Sample wound base and margins. Submit separate swab for culture to maximize recovery.
Rejection Criteria
Reject if specimen container is unlabeled or leaking.
Reject for inappropriate storage or transport.
Storage and Transport
Store specimens from sterile sources at room temperature.
Store specimens from non-sterile sources at 2-8 C.
Transport within 24 hours.
Testing Schedule
Performed upon receipt
Reference Range
No organisms seen
OR
Descriptive report: 1+ (rare), 2+ (few), 3+ (moderate), or 4+ (many) organisms and/or cellular material / field
Testing Department
Microbiology
Method
Microscopic examination
CPT Code
87205
STAT Turnaround Time
60 minutes
Routine Turnaround Time
3 hours